Impact of a modified implant macrogeometry on biomechanical parameters and bone-related markers in rats

Abstract This study investigated the impact of a modified implant macrogeometry on peri-implant healing and its effect on bone-related molecules in rats. Eighteen rats received one implant in each tibia: the control group received implants with conventional macrogeometry and the test group received implants with modified macrogeometry. After 30 days, the implants were removed for biomechanical analysis and the bone tissue around them was collected for quantifying gene expression of OPN, Runx2, β-catenin, BMP-2, Dkk1, and RANKL/OPG. Calcein and tetracycline fluorescent markers were used for analyzing newly formed bone at undecalcified sections of the tibial implants. These fluorescent markers showed continuous bone formation at cortical bone width and sparse new bone formed along the medullary implant surface in both groups. However, higher counter-torque values and upregulation of OPN expression were achieved by test implants when compared to controls. The modified macrogeometry of implants optimized peri-implant healing, favoring the modulation of OPN expression in the osseous tissue around the implants.

Does resveratrol favor peri-implant bone repair in rats with ovariectomy-induced osteoporosis? Gene expression, counter-torque and micro-CT analysis

Abstract This study investigated the influence of resveratrol on peri-implant repair and its effects on bone-related markers in ovariectomy-induced osteoporosis in rats. Animals were divided into: OVX+PLAC (n = 10): ovariectomized animals treated with placebo; OVX+RESV (n = 10): OVX treated with resveratrol; OVX+PLAC+ZOL (n = 10): OVX treated with PLAC and zoledronate; OVX+RESV+ZOL (n = 10): OVX treated with RESV and ZOL; and SHOVX+PLAC (n = 10): sham ovariectomy treated with PLAC. RESV and PLAC were administrated after ovariectomy and ZOL after six weeks after OVX, until the end of experiment. One implant was inserted in each tibiae of animals 18 weeks after ovariectomy. After 4 weeks, one implant was removed for counter-torque, and peri-implant tissue was collected for mRNA quantification of several osteogenic markers by PCR. The other tibia was submitted to micro-computed tomography analysis. Reduced counter-torque values, bone-implant contact (BIC) and bone volume fraction (BV/TV), and higher bone porosity (BP) were detected in OVX+PLAC group when compared to SHOVX+PLAC (p < 0.05). OVX+RESV rats presented lower BIC, BV/TV, and trabecular number (Tb.N), and augmented BP and trabecular spacing (Tb.Sp) when compared to SHOVX+PLAC (p < 0.05). Higher Tb.N and connectivity density (Conn.Dn) and reduced Tb.Sp were observed in OVX rats treated with ZOL, independently of RESV, when compared to OVX+PLAC and OVX+RESV groups (p < 0.05), whereas the combination ZOL+RESV promoted lower BP when compared to OVT+PLAC and OVX+RESV (p < 0.05). Gene expression was not influenced by RESV (p > 0.05), whereas ZOL promoted up-regulation of BMP-2 (p<0.05). RESV did not improve peri-implant bone repair in rats with ovariectomy-induced osteoporosis.

Association of rs142548867 (EEFSEC) and periodontitis Grade C in a young Brazilian population

Abstract Periodontitis Stage III-IV, Grade C (PerioC) is a severe form of Periodontitis. The individual genetic background has been shown to be an important etiopathogenic factor for the development of this disease in young, systemically healthy, and non-smokers patients. Recently, after exome sequencing of families with a history of the disease, PerioC was associated with three single nucleotide variations (SNVs) - rs142548867 (EEFSEC), rs574301770 (ZNF136), and rs72821893 (KRT25) - which were classified as deleterious or possibly harmful by prediction algorithms. Objective Seeking to validate these findings in a cohort evaluation, this study aims to characterize the allele and genotypic frequency of the SNVs rs142548867, rs574301770, and rs72821893 in the Brazilian population with PerioC and who were periodontally healthy (PH). Methodology Thus, epithelial oral cells from 200 PerioC and 196 PH patients were harvested at three distinct centers at the Brazilian Southern region, their DNA were extracted, and the SNVs rs142548867, rs574301770, rs72821893 were genotyped using 5′-nuclease allelic discrimination assay. Differences in allele and genotype frequencies were analyzed using Fisher's Exact Test. Only the SNV rs142548867 (C > T) was associated with PerioC. Results The CT genotype was detected more frequently in patients with PerioC when compared with PH subjects (6% and 0.5% respectively), being significantly associated with PerioC (odds ratio 11.76, p=0.02). Conclusion rs142548867 represents a potential risk for the occurrence of this disease in the Brazilian population.

Influence of rs6667202 SNP on Interleukin-10 levels in the gingival fluid of patients with periodontitis grade C

Grade C periodontitis in youngers is characterized by a severe form of periodontitis, and IL10 rs6667202 single nucleotide polymorphism (SNP) has been described as an important feature in this disease etiology. Aim: This study aimed to evaluate, in vivo, the functionality of IL10 rs6667202 SNP on IL-10 gingival fluid levels. Methods: Thirty patients with Perio4C were selected, 15 with the IL10 AA genotype (rs6667202) and 15 with AC/CC genotypes. The gingival fluid was collected from two sites with probing depth ≥ 7 mm and bleeding on probing, and two healthy sites. The IL-10 concentration was determined by Luminex/MAGpix platform. Results: In deep pockets, the IL10 AA genotype presented a lower concentration of IL-10 when compared with AC or CC genotypes (p<0.05). In shallow pockets, no difference between groups was seen (p>0.05). Conclusion: IL10 rs6667202 SNP decreases the production of IL-10 in crevicular fluid, potentially affecting this disease progression

Rethinking the decision-making process to treat gingival recession associated with non-carious cervical lesions

Abstract The presence of a tooth-surface defect, such as a non-carious cervical lesion (NCCL), associated with sites of gingival recession (GR) defects creates a combined soft tissue/tooth defect (CD) that requires a different treatment plan. This study aimed to critically review the literature regarding the available treatment protocols for CDs and suggest a new decision-making process. NCCLs were classified as Class A-: the cementoenamel junction (CEJ) was visible and the root surface discrepancy was < 0.5 mm (no step); Class A+: CEJ was visible and the root surface discrepancy was > 0.5 mm (with a step); Class B-: unidentifiable CEJ without a step; Class B+: unidentifiable CEJ with a step. NCCLs affecting both root and crown surfaces (Class B) lead to CEJ destruction and consequently eliminate an important landmark used before and after root coverage procedures. The depth of the root surface discrepancy is vital owing to its possible impact on soft tissue adaptation after healing, which, in turn, may influence the treatment options, namely the use of graft and/or composites to compensate for the discrepancy. Clinically, a step with horizontal depth greater than 0.5 mm should be recognized as the minimum threshold value to define this condition. Extremely deep defects tend to assume a V-shaped topography. Therefore, extremely deep V-shaped defects were classified into subclasses A+V, a V-shaped defect, and B+V, a V-shaped defect with loss of CEJ, for management considerations. The treatment options, supported by the literature, and a decision-making process to deal with each condition are presented.

Impact of history of periodontitis on gene expression of bone-related factors in young patients

Abstract Although dental implants and bone regenerative procedures are important approaches for the reestablishment of esthetics and function in young patients with a history of generalized aggressive periodontitis (GAP), no predictable outcomes have been reported, and the host osteo-immunoinflammatory response may play a relevant role in this context. In view of the lack of molecular investigations into the bone tissue condition of young patients with periodontitis, the aim of this study was to evaluate the gene expression of bone-related factors in this population. Bone biopsies were obtained from the posterior mandible in 16 individuals previously diagnosed with GAP and on periodontal support therapy and from 17 periodontally healthy (PH) patients. The gene expression of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), osteocalcin (OC), bone sialoprotein (BSP), and type I collagen (COL-I), important biomarkers of bone turnover, was evaluated by qRT-PCR. Lower TGF-β and OPG mRNA levels were observed in GAP patients compared to PH individuals (p ≤ 0.05). There were no between-group differences in levels of TNF-α, BSP, RANKL, OC, or COL-I mRNA (p>0.05). In young adults, a history of periodontal disease can negatively modulate the gene expression of important bone-related factors in alveolar bone tissue. These molecular outcomes may contribute to the future development of therapeutic approaches to benefit bone healing in young patients with history of periodontitis via modulation of osteo-immuno-inflammatory biomarkers.

Isolation and characterization of a human cementocyte-like cell line, HCY-23

Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.

Host response and peri-implantitis

Abstract Considering the absence of predictable and effective therapeutic interventions for the treatment of peri-implantitis, scientific evidence concerning the host response profile around dental implants could be important for providing in the future a wider preventive and/or therapeutic window for this peri-implant lesion, indicating biomarkers that provide quantifiable measure of response to peri-implant therapy. Moreover, a better knowledge of pattern of host osteo-immunoinflammatory modulation in the presence of peri-implantitis could either benefit the early diagnostic of the disease or to cooperate to prognostic information related to the status of the peri-implant breakdown. Finally, new evidences concerning the host profile of modulators of inflammation and of osseous tissue metabolism around dental implants could explain the individual susceptibility for developing peri-implant lesions, identifying individuals or sites with increased risk for peri-implantitis. The focus of this chapter was, based on a systematically searched and critically reviewed literature, summarizing the existing knowledge in the scientific research concerning the host osteo-immunoinflammatory response to the microbiological challenge related to periimplantitis.

Periodontal disease and associated factors in the adult and elderly population from Jundiaí City, Brazil

Aim: This study assessed the prevalence of periodontal disease in the adult and elderly populations from Jundiaí City, and its association with individual social inequalities in a conceptual framework approach. Methods: The survey was conducted with a sample of 342 adults and 145 elderly, and periodontal disease was assessed based on the Community Periodontal Index (CPI) and Clinical Attachment Loss (CAL). A questionnaire addressing socio-demographic and behavioral variables, smoking and diabetes was included. Bivariate and multivariate analyses, using binary regression analyses, were carried out in a hierarchical approach with conceptual framework to reveal association among periodontal disease and social-demographic, smoking and diabetes variables. Results: One adult and fifty-six elderly who had lost all teeth were excluded from the sample. Mild periodontal disease (CAL ≤3 mm) was the condition more prevalent in the adult (74%) and elderly populations (60.6%). Adjusted analysis revealed that low educational level (OR 2.65, 95% CI 1.19-5.88), irregular use of tooth floss (OR 1.9, 95% CI 1.06-3.40), and smoking (OR 2.14, 95% CI 1.04-4.42) were independently associated with moderate/severe periodontal disease (CAL and Probing Depth ≥4 mm) in the adult group. For the elderly group, low educational levels (OR 0.16, 95% CI 0.04-0.58), use of public dental service (OR 5.32, 95% CI 1.23-23.03), and diabetes condition (OR 3.78, 95% CI 1.20-11.91) were significantly associated with periodontal disease. Conclusion: In conclusion, the data showed that education level, smoking habits, diabetes, use of dental floss and type of dental service are factors associated to moderate/severe periodontal disease among Brazilians from Jundiaí City

Doença periodontal e artrite idiopática juvenil: uma inter-relação bidirecional / Periodontal disease and juvenile idiopathic arthritis: a bidirectional inter-relationship

Parece haver similaridades entre a patogenia de doenças reumatológicas (artrite reumatoide e artrite idiopática juvenil) e periodontite. Alguns estudos têm sido conduzidos com o objetivo de elucidar os mecanismos que explicam a inter-relação entre essas condições. A artrite reumatoide parece aumentar a suscetibilidade à doença periodontal destrutiva em adultos e em pacientes com artrite idiopática juvenil. No entanto, ainda são pouco conhecidas as vias de associação entre essas condições crônicas infl amatórias (periodontite e artrite). Desta forma, o objetivo deste trabalho foi promover uma revisão da literatura sobre a inter-relação artrite idiopática infantil e doença periodontal.

Similarities between rheumatologic diseases (rheumatoid arthritis and juvenile idiopathic arthritis) and periodontitis pathogenesis are discussed. Some studies have been conducted to elucidate the mechanisms that explain the relationship between these conditions. Rheumatoid arthritis appears to increase susceptibility to destructive periodontal disease in adults and in patients with juvenile idiopathic arthritis. However, the pathway of association between these chronic infl ammatory conditions are barely known. Thus, the aim of this study was to review the literature concerning the relationship between juvenile idiopathic arthritis and periodontitis.

Mesenchymal stem cells in periodontics: new perspectives / Células mesenquimais indiferenciadas em periodontia: novas perspectivas

ABSTRACT Tissue engineering is a contemporary field of science, which aims to create conditions based on principles of cell and molecular biology, bioengineering and biomaterials to regenerate tissues. Mesenchymal stem cells present high proliferation rates and are able to differentiate into multilineages under certain conditions, suggesting that they have great potential to act in regeneration field. Tooth derived stem cells are a suitable alternative source of mesenchymal cells once they are easily accessible and have poor morbidity to the donor. Studies showed that they have been isolated and characterized from diverse tissues such as dental pulp, exfoliated deciduous teeth, periodontal ligament, gingiva, dental follicle and apical papilla. However studies show that there is heterogeneity among these populations and there is no standard method to select the most appropriate tooth derived stem cells for regenerative procedures. The aim of this review is to present the current perspective of the multiple types of tooth-derived stem cells and to discuss the basis for their use in periodontal tissue engineering.

RESUMO A engenharia de tecidos é um campo contemporâneo da ciência, que visa criar condições baseadas em princípios de biologia celular e molecular, bioengenharia e biomateriais para regenerar tecidos. As células tronco mesenquimais apresentam altas taxas de proliferação e são capazes de se diferenciar, sob certas condições, em multi-linhagens, sugerindo que elas têm grande potencial para atuar no campo da regeneração. As células tronco derivadas de tecidos dentais são uma fonte alternativa adequada de células mesenquimais uma vez que são de fácil acesso e têm baixa morbidade para o doador. Estudos demonstraram que elas já foram isoladas e caracterizadas a partir de diversos tecidos tais como polpa dentária, dentes decíduos esfoliados, ligamento periodontal, gengiva, folículo dental e papila apical. Entretanto, os estudos demonstram que há heterogeneidade entre essas populações e não existe um método padrão para selecionar as células-tronco dentais mais apropriadas para procedimentos regenerativos. O objetivo desta revisão é apresentar o conhecimento atual dos vários tipos de células-tronco derivadas de dentes e discutir as novas perspectivas para seu uso na engenharia de tecidos periodontais.

Cemento dental: aspectos atuais de interesse para o clínico / Dental cementum: current aspects to the clinicians

Embora tenha havido avanço no entendimento da homeostase do cemento dental, o papel deste tecido e sua biologia permanecem não completamente elucidados. Este estudo buscou fornecer informações sobre os conhecimentos mais recente relacionados à biologia do cemento dental, com o objetivo de discutir o papel exercido por este tecido em condições não fisiológicas nos tecidos periodontais. Devido aos avanços na exploração do tecido ósseo, que compartilha diversas características similares, a pesquisa abrangente sobre o cemento dental tem sido encorajada, a fim de esclarecer a função completa deste tecido na homeostase periodontal e regeneração. Desta forma, no presente trabalho, sempre que possível será feito um paralelo entre osso alveolar e cemento dental. O desenvolvimento de metodologias e técnicas celulares e moleculares avançadas possibilitou um melhor entendimento do comportamento do cemento em situações diversas, como quando em situações patológicas, como a doença periodontal, e até mesmo frente à regeneração tecidual. Ademais, estudos clínicos e em modelo animal demonstraram resultados em relação à formação de cemento em abordagens regenerativas. No entanto, sugere-se que estudos posteriores possam contribuir para um melhor conhecimento sobre o cemento e o perfil celular dos cementoblastos e cementócitos, bem como suas interações para fornecer novos insights para o desenvolvimento de terapias eficientes e mais previsíveis para regeneração dos tecidos periodontais. Apesar dos avanços dos estudos clínicos e laboratoriais, pôde-se concluir que inúmeras questões referentes à biologia do cemento permanecem não esclarecidas.

Although some progress has been made to understand dental cementum homeostasis, its role and biology remains not completely elucidated. This study aimed to provide information on the recent knowledge related to the dental cementum biology, in order to discuss the role of this tissue in physiological and non-physiological conditions in the periodontal tissues. Due to advances in the exploration of bone tissue, which shares several similar features, comprehensive research on dental cementum has been encouraged in order to clarify the complete function of this tissue in periodontal homeostasis and regenerative approach. Novel methodologies and advanced cellular and molecular techniques provided better understanding of cementum in different circumstances, as pathological situations such as periodontal disease and even tissue regeneration. In addition, clinical and animal model designs show positive outcomes to cementum formation in regenerative approaches, however, it is suggested that further studies may contribute to better understand cementum tissue and cementoblasts and cementocytes profile, as well as their interactions, providing new insights to develop efficient and more predictable therapies for periodontal tissue regeneration. Despite advances in clinical and laboratory studies, it can be concluded that many questions regarding the cementum biology remain unclear.

Aumento de coroa estético associado à reabilitação protética ­ relato de caso clínico / Aesthetic crown lengthening associated with prosthetic rehabilitation ­ a case report

Atualmente, a estética periodontal ou estética vermelha tem sido muito discutida e valorizada na busca pela harmonia do sorriso. Em situações de erupção passiva alterada, ou seja, existência de grande exposição de gengiva acompanhada de coroas clínicas curtas, há uma indicação precisa para a execução de aumento de coroa clínica. A Odontologia estética reabilitadora exerce um papel fundamental no tratamento multidisciplinar. Este trabalho teve como objetivo relatar um caso clínico de sorriso gengival causado pela erupção passiva alterada, associando reabilitação estética com facetas dentárias. Após a conclusão do tratamento, foi possível afirmar que a interação Prótese/Periodontia foi de fundamental importância para a obtenção do resultado final esperado pela paciente.

Nowadays, the periodontal or red esthetics has been highlightened in the search of smile harmony. In cases of altered passive eruption, which means the existence of great gum exhibition and short clinical crowns, there is precise indication for crown lengthening. Esthetic dentistry has a fundamental role in the multidisciplinary treatment. This paper reports on a clinical case of gummy smile due to passive eruption receiving an oral rehabilitation with laminate veneers. After treatment delivery, it was possible to recognize that the Prosthodontic/Periodontal relationship is of critical importance for the patient's desired outcome.

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Abstract Periodontitis develops as a result of a continuous interaction between host cells and subgingival pathogenic bacteria. The periodontium has a limited capacity for regeneration, probably due to changes in periodontal ligament stem cells (PDLSCs) phenotype. The aim of this study was to evaluate the effects of lipopolysaccharides from Porphyromonas gingivalis (PgLPS) on mesenchymal phenotype and osteoblast/cementoblast (O/C) potential of PDLSCs. PDLSCs were assessed for Toll-like receptor 2 (TLR2) expression by immunostaining technique. After, cells were exposed to PgLPS, and the following assays were carried out: (i) cell metabolic activity using MTS; (ii) gene expression for IL-1β, TNF-α and OCT-4 by real-time polymerase chain reaction (RT-qPCR); (iii) flow cytometry for STRO-1 and CD105, and (iv) osteogenic differentiation. PDLSCs were positive for TLR2. PgLPS promoted cell proliferation, produced IL-1β and TNF-α, and did not affect the expression of stem cell markers, STRO-1, CD105 and OCT-4. Under osteogenic condition, PDLSCs exposed to PgLPS showed a similar potential to differentiate toward osteoblast/cementoblast phenotype compared to control group as revealed by mineralized matrix deposition and levels of transcripts for RUNX2, ALP and OCN. These results provide evidence that PgLPS induces pro-inflammatory cytokines, but does not change the mesenchymal phenotype and osteoblast/cementoblast differentiation potential of PDLSCs.

Técnica de enxerto gengival livre para aumento de tecido queratinizado em recessão gengival classe III de Miller / Free gingival graft technique for keratinized tissue increase in Miller III gingival recession ­ a case report

A presença ou ausência de gengiva inserida pode interferir na manutenção da saúde gengival, uma vez que sua deficiência leva à exposição radicular dificultando o processo de higienização, o que resulta na presença de biofilme e inflamação gengival com perda de inserção. Foi realizada terapia periodontal cirúrgica com enxerto gengival livre em paciente com recessão gengival classe III de Miller para aumento de faixa de tecido queratinizado. Após período de pós-operatório e acompanhamento de um ano e 12 meses, foi observado ganho e manutenção de volume e altura do tecido queratinizado enxertado, com saúde gengival e ganho de inserção. Nos casos onde há necessidade de reforçar áreas fragilizadas pela diminuição ou falta de gengiva inserida, a técnica de enxerto gengival livre apresenta boa previsibilidade e manutenção da faixa de tecido queratinizado, favorecendo o controle de biofilme e saúde gengival.

The presence or absence of attached gingiva may interfere with the maintenance of gingival health, since its defi ciency in some cases complicates the process of cleaning, resulting in the presence of biofilm and gingival inflammation with insertion loss. This case report describes a surgical technique based on free gingival graft in a patient with gingival recession Miller class III to increase keratinized tissue band. After the postoperative period of 12 months tissue improvement was observed, as well as, volume maintenance and height of keratinized grafted tissue along with gum health and insertion gain. In cases where there is need to strengthen weakened areas by reducing or lack of attached gingiva, the free gingival graft technique has good predictability and maintenance of keratinized tissue range, favoring the control of biofilm and gingival health.

Transmissão intrafamiliar de patógenos periodontais / Intrafamilial transmission of periodontal pathogens: a literature review

Objetivo: revisar quais seriam os diferentes fatores envolvidos na transmissão de periodontopatógenos entre membros de uma mesma família e quais as suas consequências. Material e métodos: uma revisão da literatura foi realizada na base de dados PubMed, utilizando os termos "vertical transmission", "periodontal pathogens", "oral colonization", e "periodontitis". Resultados: após a leitura do título e resumo, 30 artigos foram incluídos nesta revisão. A transmissão de patógenos periodontais entre indivíduos de uma mesma família está relacionada à passagem via salivar e ao compartilhamento alimentar e de higiene, aos cuidados dos filhos pelos pais ou cuidadores, e ao contato íntimo entre cônjuges. Estudos que avaliaram a transmissão do Aggregatibacter actinomycetemcomitans entre indivíduos de uma mesma família mostraram a ocorrência da transmissão vertical, embora também ocorra transmissão horizontal. Entretanto, resultados semelhantes não puderam ser observados para o Porphyromonas gingivalis. Enquanto alguns relatos indicam a ocorrência de transmissão horizontal desta bactéria, diversos outros estudos indicam características bacterianas que reduzem sua ocorrência. Conclusão: a colonização oral por microrganismos patogênicos está relacionada à transmissão vertical e horizontal de patógenos, embora a persistência dos microrganismos pareça estar relacionada a fatores individuais do hospedeiro e características dos patógenos. Além disso, atividades preventivas e terapêuticas devem ser realizadas de forma a alterar o processo de transmissão, colonização e o maior risco do desenvolvimento de problemas periodontais.

Objective: to review the different factors involved in the transmission of periodontopathogens between members of the same family and their consequences. Material and methods: an electronic literature review was conducted at the PubMed using the keywords "vertical transmission", "periodontal pathogens", "oral colonization", and "periodontitis". Results: after reading of title and abstract, 30 articles were included. The transmission of periodontal pathogens among individuals of the same family is related to the passage through salivary and food and hygiene sharing, the care of the children by parents or caregivers, and the intimate contact between individuals. Studies evaluating the transmission of Aggregatibacter actinomycetemcomitans among individuals from the same family showed the occurrence of vertical transmission and horizontal transmission. However, similar results could not be observed for Porphyromonas gingivalis. While some reports indicate the occurrence of horizontal transmission, several other studies indicate bacterial characteristics that reduce its occurrence. Conclusion: oral colonization by pathogenic microorganisms is related to its vertical and horizontal transmission, although the persistence of the microorganisms seems to be related to individual host factors and pathogen characteristics. In addition, preventive and therapeutic activities must be performed in a way that will alter the transmission, colonization and the greater risk of developing periodontal problems.

Regeneração óssea guiada no tratamento de peri-implantite: dois anos de acompanhamento clínico / Guided bone regeneration in the treatment of peri-implantitis: 2 years of clinical follow-up

A peri-implantite é caracterizada pelo processo inflamatório ao redor de um implante, que inclui inflamação do tecido mole e perda progressiva de suporte ósseo. O foco do seu tratamento está no controle da infecção bacteriana, através da eliminação do biofilme da superfície do implante e, quando possível, na regeneração do osso perdido. Contudo, atualmente não existe evidência suficiente na literatura para indicar qual abordagem terapêutica é superior no tratamento da peri-implantite a longo prazo. Dessa forma, o objetivo do presente relato de caso clínico foi reportar os resultados a longo prazo do tratamento de um caso de peri-implantite, com a combinação de acesso cirúrgico para descontaminação da superfície do implante, uso adjunto de solução de tetraciclina (50 mg/ml) e procedimento de regeneração óssea guiada, utilizando enxerto xenógeno e membrana reabsorvível de colágeno. Aos dois anos de acompanhamento pós-operatório, observou-se uma melhora clínica significativa, com redução da profundidade de sondagem, ganho de inserção clínica e preenchimento ósseo radiográfico do defeito peri-implantar. Pôde-se observar que o acesso cirúrgico associado à regeneração óssea guiada é uma alternativa viável para o tratamento de lesões de peri-implantite.

Peri-implantitis is an inflammatory process around an implant, which includes soft tissue inflammation and progressive bone loss. Treatment aims to control the bacterial infection through elimination of the established biofilm from the implant surface, and if possible, the regeneration of the lost bone. However, currently there is not sufficient evidence in the literature supporting which therapeutic approach is most suitable for the treatment of peri-implantits at long-term follow-up. Thus, the aim of this case demonstrate was to report the results of a peri-implantitis treatment at long-term follow-up, with the combination of surgical access for decontamination of the implant surface, with adjunctive use of tetracycline solution (50 mg/ml) and guided bone regeneration procedure using xenogeneic graft and resorbable collagen membrane. A significant clinical improvement was observed at 2 years of follow-up, with reduced probing depth, clinical attachment gain and radiographic bone fill in the peri-implant defect. It can be concluded that the surgical access associated with guided bone regeneration is a viable option for the treatment of peri-implant lesions

Impact of micronutrients supplementation on bone repair around implants: microCT and counter-torque analysis in rats

ABSTRACT The use of natural substances and micronutritional approaches has been suggested as a therapeutic alternative to benefit the bone healing associated with no side effects. Nevertheless, the influence of micronutritional interventions with therapeutic proprieties on the bone repair has yet to be intensely evaluated, and no evidence is available exploring the impact of micronutrient supplementation on the peri-implant bone healing. Objective This study investigated the effect of micronutrients supplementation on the bone repair around implants. Material and Methods One screw-shaped titanium implant was inserted in each tibia of each rat, which were assigned to: daily administration, for 30 d, of the placebo solution (Placebo group-n:18) or micronutrients supplementation (Micronutrients group-n:18), based on calcium, magnesium, zinc, and vitamin D3 intake. After, the animals were sacrificed. One of the implants was removed by applying a counter-torque force to evaluate the force to rupture the bone-implant interface. The other implant was evaluated by microcomputed tomography (CT) examination to determine the bone-to-implant contact (BIC) and the bone volume (BV/TV). Results No statistically significant differences were observed between the groups for both counter-torque values and microCT parameters (p>0.05). Conclusion Within the limits of this study, micronutrients supplementation did not provide additional benefits to the bone healing around dental implants.

Maternal periodontitis induces intergenerational injuries on reproductive performance of adult female rat offspring / Periodontite materna induz prejuízos transgeracionais no desempenho reprodutivo da prole feminina na idade adulta

Substantial evidence suggests a direct link between periodontitis in pregnant women and subsequent adverse pregnancy outcomes. However, no studies have evaluated the transgenerational effects of periodontitis on the reproductive performance of subsequent generations. The present study investigated whether maternal periodontal disease exerts deleterious transgenerational effects on reproductive performance in F1 female rats. Rat female offspring from mothers that were subjected to experimentally induced periodontitis or sham operation were mated with sexually experienced male rats. The weight and reproductive performance of these F1 offspring were evaluated on gestation day 21, including maternal weight, litter weight, individual pup weight, number of pups, and number of resorptions. The percentage of dams with resorptions and the litter weight/number of pups were also calculated. Compared with the control group, an increase was observed in the percentage and number of resorptions and litter weight/number of pups, and a decrease was observed in the number of pups born in the experimental group. Maternal weight, litter weight, and individual pup weight were not different between the control and experimental groups. Maternal periodontitis impaired reproductive performance in the F1 generation. We showed that periodontitis may induce reproductive injury in adult offspring even if the offspring do not undergo any inflammatory/infectious process during their postnatal life or during gestation. These findings reinforce the importance of oral care during pregnancy.(AU)

Existem evidências substanciais de uma relação direta entre periodontite em mulheres grávidas com efeitos adversos reprodutivos. No entanto, nenhum estudo avaliou os efeitos intergeracionais da periodontite sobre o desempenho reprodutivo das gerações subsequentes. O presente estudo investigou se a doença periodontal materna exerce efeitos intergeracionais deletérios sobre o desempenho reprodutivo em ratos fêmeas da geração F1. Assim, filhas de ratas cujas mães foram submetidas a periodontite experimental ou falsamente operadas foram acasaladas com ratos machos sexualmente experientes. O peso corporal e desempenho reprodutivo da geração F1 foram avaliados no dia 21 de gestação, incluindo o peso materno, peso da ninhada, peso da individual dos filhotes, número de filhotes e de reabsorções. A percentagem de fêmeas com reabsorção e o peso da ninhada/número de filhotes também foram calculados. Comparados com o grupo controle, observou-se aumento na porcentagem e número de reabsorções e no peso da ninhada/ número de filhotes, e decréscimo no número de filhotes nascidos no grupo experimental. O peso materno, peso da ninhada e individual dos filhotes não foi diferente entre o controle e experimental. Estes resultados mostram que a periodontite experimental materna prejudica o desempenho reprodutivo da geração F1, mesmo que estes animais não tenham sido expostos diretamente a um processo inflamatório.(AU)

Effect of Resveratrol on periodontal pathogens during experimental periodontitis in rats

Abstract The objective of this study was to investigate the antibacterial effect of resveratrol against putative periodontal pathogens during the progression of experimental periodontitis in rats. Periodontitis was induced in rats in one of the first molars chosen to receive a ligature. Animals were assigned to one of two groups: daily administration of the placebo solution (control group, n = 12) or 10 mg/Kg of resveratrol (RESV group, n = 12). The therapies were administered systemically for 30 days, for 19 days before periodontitis induction and then for another 11 days. Then, the presence and concentrations of Porphyromonas gingivalis, Tannerella forsythia and Aggregatibacter actinomycetemcomitans in the cotton ligatures collected from the first molars were evaluated using real-time PCR. Inter-group comparisons of the microbiological outcomes revealed that no differences were detected for P. gingivalis, T. forsythia and A. actinomycetemcomitans levels (p > 0.05). Continuous use of resveratrol did not promote additional benefits in microbiological outcomes during experimental periodontitis in rats.